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KMID : 1140120090140020153
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Cancer Prevention Research
2009 Volume.14 No. 2 p.153 ~ p.160
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Induction of G1 Arrest of Cell Cycle by Ethyl Alcohol Extract of Hizikia fusiforme in Human Breast Carcinoma Cells
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Jung Sun-Hwa
Choi Yung-Hyun
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Abstract
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Hizikia fusiforme is a kind of brown edible seaweed that mainly grows in the northwest pacific including Korea, Japan and China, and has been widely used as a healthy food for hundreds of years. Recently, H. fusiforme has been known to exert pharmacological activities including antioxidant, antimutagenic and anticoagulant activity. However, the molecular mechanisms of H. fusiforme in malignant cells have not been clearly elucidated yet. In this study, it was investigated the effects of ethyl alcohol extract of H. fusiforme (EAHF) on the anti-proliferative effects of MDA-MB-231 and MCF-7 human breast cancer cells. EAHF treatment resulted in a concentration-dependent growth inhibition by including G1 phase arrest of the cell cycle in MDA-MB-231 and MCF-7 cells as measured by flow cytometry analysis. The G1 arrest of EAHF was accompanied by an inhibition of cyclin D1 and cyclin-dependent kinase 2 (Cdk2) expression. EAHF treatment induced the level of Cdk inhibitor p27 without significant alteration of p53 and p21 expression. However, EAHF treatment resulted in a down-regulation of retinoblastoma protein (pRB) and E2F-1 and E2F-4 expression. In addition, the cell motility of both cell lines was markedly inhibited by EAHF treatment in a time- and concentration-dependent manner. The present results suggest that EAHF has divergent biological functions and additional studies will be needed to identify the active compounds that confer the anti-cancer activity of EAHF.
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KEYWORD
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Hizikia fusiforme, Breast cancer cells, G1 arrest
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